Sequence analysis of an HIV-1 isolate which displays unusually high-level AZT resistance in vitro.

@article{Muckenthaler1992SequenceAO,
  title={Sequence analysis of an HIV-1 isolate which displays unusually high-level AZT resistance in vitro.},
  author={Martina Muckenthaler and Nikolas Gunkel and P. Levantis and Kate Broadhurst and Beng Hwee Goh and Bruce A. Colvin and Geoffrey Forster and George Gee Jackson and John S. Oxford},
  journal={Journal of medical virology},
  year={1992},
  volume={36 2},
  pages={
          79-83
        }
}
Multiple mutations in the reverse transcriptase (RT) gene were observed in a drug-resistant isolate of human immunodeficiency virus type 1 (HIV1) from an individual having prolonged (greater than 2 years) zidovudine (AZT) therapy. The virus replicated in PBMC's in the presence of very high concentrations of AZT (125 microM). Drug-sensitive strains were curtailed by 0.01 microM AZT. Eleven defined mutations were observed as compared with published sequences of RT for eight strains of HIV1. Eight… CONTINUE READING