Sequence analysis of a rainbow trout cDNA library and creation of a gene index.

@article{Rexroad2003SequenceAO,
  title={Sequence analysis of a rainbow trout cDNA library and creation of a gene index.},
  author={Caird E. Rexroad and Yi-Hsuan Lee and John W. Keele and Svetlana Karamycheva and Garth G Brown and Ben F. Koop and Scott A. Gahr and Yniv Palti and John Quackenbush},
  journal={Cytogenetic and genome research},
  year={2003},
  volume={102 1-4},
  pages={347-54}
}
Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large-scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 5' ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index… CONTINUE READING
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