Sequence analysis by hybridization with oligonucleotide microchip: identification of beta-thalassemia mutations.

@article{Drobyshev1997SequenceAB,
  title={Sequence analysis by hybridization with oligonucleotide microchip: identification of beta-thalassemia mutations.},
  author={Alexei L. Drobyshev and N V Mologina and Venyamin Shik and D D Pobedimskaya and Gennadiy Yershov and Andrei Mirzabekov},
  journal={Gene},
  year={1997},
  volume={188 1},
  pages={45-52}
}
Diagnostics for genetic diseases were run and sequence analysis of DNA was carried out by hybridization of RNA transcripts with oligonucleotide array microchips. Polyacrylamide gel pads (100 x 100 x 20 microm) were fixed on a glass slide of the microchip and contained allele-specific immobilized oligonucleotides (10-mers). The RNA transcripts of PCR-amplified genomic DNA were fluorescently labeled by enzymatic or chemical methods and hybridized with the microchips. The simultaneous measurement… CONTINUE READING

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