Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis

  title={Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis},
  author={Larry A. Gallagher and Elizabeth R. Ramage and Rapatbhorn Patrapuvich and Eli Weiss and Mitch J. Brittnacher and Colin S Manoil},
UNLABELLED We constructed a near-saturation transposon mutant library for Burkholderia thailandensis, a low-virulence surrogate for the causative agent of melioidosis (Burkholderia pseudomallei). A primary set of nearly 42,000 unique mutants (~7.5 mutants/gene) was generated using transposon Tn5 derivatives. The strains carry insertions in 87% of the predicted protein-coding genes of the organism, corresponding to nearly all of those nonessential for growth on nutrient agar. To achieve high… CONTINUE READING
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