Sequence Characteristics of Natural Populations of Tomato Spotted Wilt Tospovirus Infecting Flue-cured Tobacco in Georgia

@article{Pappu1998SequenceCO,
  title={Sequence Characteristics of Natural Populations of Tomato Spotted Wilt Tospovirus Infecting Flue-cured Tobacco in Georgia},
  author={Hanu R. Pappu and Sita S. Pappu and Rakesh Jain and Paul Bertrand and Albert Culbreath and Robert Mcpherson and Alex S. Csinos},
  journal={Virus Genes},
  year={1998},
  volume={17},
  pages={169-177}
}
Using primers from conserved regions, the nucleocapsid (Nc) gene sequences of naturally occurring tomato spotted wilt tospovirus (TSWV) isolates from flue-cured tobacco (Nicotiana tabacum) grown in several Georgia counties were amplified by immunocapture-reverse transcription-polymerase chain reaction. The resulting amplicons were cloned and sequenced. Sequence analyses showed a high degree of sequence conservation among the Nc genes of the tobacco isolates, and those reported from other parts… CONTINUE READING

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