Sequence Analysis and Detection of Ralstonia solanacearum by Multiplex PCR Amplification of 16S–23S Ribosomal Intergenic Spacer Region with Internal Positive Control

Abstract

Polymerase chain reaction (PCR) methods for detection and differentiation of Ralstonia solanacearum strains were compared. The 16S–23S rRNA gene ITS sequence data revealed the two main sequence clusters (divisions I and II) of R. solanacearum and further subclusters of division II. Based on this sequence data, primers were designed which differentiated… (More)
DOI: 10.1023/A:1021218201771

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@article{Pastrik2004SequenceAA, title={Sequence Analysis and Detection of Ralstonia solanacearum by Multiplex PCR Amplification of 16S–23S Ribosomal Intergenic Spacer Region with Internal Positive Control}, author={K.-H. Pastrik and John G. Elphinstone and Rudiger Pukall}, journal={European Journal of Plant Pathology}, year={2004}, volume={108}, pages={831-842} }