Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4

@article{Versele2004SeptinCF,
  title={Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4},
  author={Matthias Versele and J. Thorner},
  journal={The Journal of Cell Biology},
  year={2004},
  volume={164},
  pages={701 - 715}
}
Assembly at the mother-bud neck of a filamentous collar containing five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) is necessary for proper morphogenesis and cytokinesis. We show that Cdc10 and Cdc12 possess GTPase activity and appropriate mutations in conserved nucleotide-binding residues abrogate GTP binding and/or hydrolysis in vitro. In vivo, mutants unable to bind GTP prevent septin collar formation, whereas mutants that block GTP hydrolysis do not. GTP binding-defective Cdc10 and Cdc12… CONTINUE READING
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