Separation of the enzyme cofactor pyrroloquinoline quinone and three isomeric analogues by capillary electrophoresis with ion-pairing media.

@article{Smith2000SeparationOT,
  title={Separation of the enzyme cofactor pyrroloquinoline quinone and three isomeric analogues by capillary electrophoresis with ion-pairing media.},
  author={A. R. Smith and Jon R Kirchhoff and Zhiwei Zhang and L M Viranga Tillekeratne and Richard A. Hudson},
  journal={Journal of chromatography. A},
  year={2000},
  volume={876 1-2},
  pages={193-9}
}
The enzyme cofactor pyrroloquinoline quinone (PQQ) was successfully separated from three closely related isomeric analogues by capillary electrophoresis with ultraviolet detection. Rapid and efficient separation of all four negatively charged isomers with baseline resolution was achieved by the addition of low concentrations (1-5 mM) of short chain tetraalkylammonium (TAA) salts to the capillary buffer. The TAA cations act as ion-pairing agents and promote differential migration of the isomers… CONTINUE READING