Separation of sperm and vaginal cells with flow cytometry for DNA typing after sexual assault.

@article{Schll1999SeparationOS,
  title={Separation of sperm and vaginal cells with flow cytometry for DNA typing after sexual assault.},
  author={Wolfgang M J Sch{\"o}ll and Michael Klintschar and Ramin Mirhashemi and Barbara Pertl},
  journal={Obstetrics and gynecology},
  year={1999},
  volume={94 4},
  pages={623-7}
}
BACKGROUND Successful DNA typing after rape is limited when only a few sperm and numerous vaginal cells are recovered from a swab, resulting in an extremely unfavorable ratio of male to female DNA. The goal of this study was to develop a protocol involving sperm cell sorting with flow cytometry based on differences in ploidy, major histocompatibility class I, CD45, and cytokeratin expression. METHOD Vaginal lavages were mixed with serially diluted ejaculate. After immunostaining and… CONTINUE READING
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