Separation of proteins on surface-modified poly(dimethylsiloxane) microfluidic devices.

@article{Dou2004SeparationOP,
  title={Separation of proteins on surface-modified poly(dimethylsiloxane) microfluidic devices.},
  author={Y Y Dou and Ning Bao and Jingjuan Xu and Fei Meng and Hong-Yuan Chen},
  journal={Electrophoresis},
  year={2004},
  volume={25 17},
  pages={3024-31}
}
Separation and detection of proteins have been realized on nonionic surfactant-modified poly(dimethylsiloxane) (PDMS) microfabricated devices with end-column amperometric detection. The hydrophobic PDMS channels are turned into hydrophilic ones after being modified with Brij35 and facilitate the separation of proteins. The coating can remarkably reduce the adsorption of large protein molecules and is stable in the range of pH 6-12. The detection of proteins in such channels needs less rinsing… CONTINUE READING