Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells

@article{Jones1990SeparationOP,
  title={Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells},
  author={Richard M. Jones and John E Wagner and Paul Celano and Milada S. Zicha and Saul J. Sharkis},
  journal={Nature},
  year={1990},
  volume={347},
  pages={188-189}
}
LONG-TERM reconstitution of the lymphohaematopoietic cells of a mouse after lethal irradiation requires the transplantation of at least (5–10) × 103 bone marrow cells1,2. Several cell-separation techniques based on cell-surface characteristics have been used in attempts to identify the pluripotent haematopoietic stem cells (PHSC), and have allowed the long-term engraftment of lethally irradiated mice with an enriched fraction of fewer than 200 marrow cells3–5. But these techniques enrich not… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 136 extracted citations

Similar Papers

Loading similar papers…