Separation of plasma carotenoids and quantitation of beta-carotene using HPLC.

@article{Gatautis1987SeparationOP,
  title={Separation of plasma carotenoids and quantitation of beta-carotene using HPLC.},
  author={Vytenis J. Gatautis and Karl H. Pearson},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1987},
  volume={166 2-3},
  pages={195-206}
}
A method was developed for the extraction and separation of human plasma carotenoids and quantitation of beta-carotene. Carotenoids were extracted from plasma with ethanol: hexane and separated by C18 reversed phase HPLC using spherical 3 micron packing. beta-Carotene was identified and quantitated using an external standard. The within-run precision of three different plasma pools ranged from 3.53-5.72% relative standard deviation (RSD). The between-run precision was 7.34% RSD. The method was… CONTINUE READING
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