Separation of phenolic acids and flavone natural antioxidants by two-dimensional method combining liquid chromatography and micellar electrokinetic capillary chromatography.

@article{esla2010SeparationOP,
  title={Separation of phenolic acids and flavone natural antioxidants by two-dimensional method combining liquid chromatography and micellar electrokinetic capillary chromatography.},
  author={Petr {\vC}esla and Jan Fischer and Pavel Jandera},
  journal={Electrophoresis},
  year={2010},
  volume={31 13},
  pages={2200-10}
}
A 2-D method was developed for separation of phenolic acids and flavone compounds combining LC with MEKC. The effect of substituted neutral and anionic CD additives to the background electrolyte on the quality of MEKC separation was investigated. The best selectivity of the MEKC separation was achieved in 25 mmol/L borate background buffer at pH 9.05 with the addition of 10 g/L SDS and 1.85 g/L heptakis (6-O-sulfo)-beta-CD. These conditions were used in the second dimension of 2-D combination… CONTINUE READING