Separation of oxidized and deamidated human growth hormone variants by isocratic reversed-phase high-performance liquid chromatography.

@article{Karlsson1999SeparationOO,
  title={Separation of oxidized and deamidated human growth hormone variants by isocratic reversed-phase high-performance liquid chromatography.},
  author={Gunnar Karlsson and P{\"a}r Gellerfors and Asa E Persson and B{\"o}rje Nor{\'e}n and Per Olof Edlund and Carin Sandberg and Staffan Birnbaum},
  journal={Journal of chromatography. A},
  year={1999},
  volume={855 1},
  pages={147-55}
}
Reversed-phase high-performance liquid chromatography (RP-HPLC) was utilized for the separation of recombinant human growth hormone (hGH) variants on a C18 silica column at 55 degrees C using an isocratic mobile phase which contained 27% 1-propanol in a 25 mM potassium phosphate buffer, pH 6.5. Three of the obtained peaks were characterized by tryptic mapping and mass spectrometry; two of the peaks were found to contain oxidized hGH (dioxy Met14/Met125 and Met125 sulfoxide) while the third… CONTINUE READING