Separation of oligomannose-type sugar chains having one to five mannose residues by high-performance liquid chromatography as their pyridylamino derivatives.

Abstract

Ten oligomannose-type sugar chains (ManGlcNAc2-Man5GlcNAc2) were prepared from various glycoproteins and fluorescence labeled with 2-aminopyridine. The fluorescent pyridylamino (PA)-sugar chains were first separated into five fractions according to their molecular sizes by HPLC on a TSK gel Amide-80 column. Each fraction was then separated into the… (More)

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Cite this paper

@article{Oku1990SeparationOO, title={Separation of oligomannose-type sugar chains having one to five mannose residues by high-performance liquid chromatography as their pyridylamino derivatives.}, author={Hiroyuki Oku and Sumihiro Hase and Tokuji Ikenaka}, journal={Analytical biochemistry}, year={1990}, volume={185 2}, pages={331-4} }