Separation of nucleosides on polyacrylamide gel columns.

@article{Carrara1968SeparationON,
  title={Separation of nucleosides on polyacrylamide gel columns.},
  author={Michele Carrara and Giorgio Bernardi},
  journal={Biochimica et biophysica acta},
  year={1968},
  volume={155 1},
  pages={
          1-7
        }
}
Abstract A new method is described for the separation of nucleosides of both the ribo-and the deoxyribo-series. Polyacrylamide gel (Bio-Gel P-2, Bio Rad, Richmond, Calif.) columns are used. At alkaline pH the elution order is I, G+T, C, A; at neutral pH the elution order is C+T, I, A, G. The unresolved fraction may be separated at the other pH. The advantages of this new separation technique are the following: recoveries from the columns are quantitative; nucleosides are eluted in very small… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-2 OF 2 CITATIONS