Separation of mono- and divinyl chlorophyll species by high-performance liquid chromatography using an octadecyl polyvinyl alcohol polymer column.

@article{Shioi1995SeparationOM,
  title={Separation of mono- and divinyl chlorophyll species by high-performance liquid chromatography using an octadecyl polyvinyl alcohol polymer column.},
  author={Yuzo Shioi and Koju Watanabe and Ken'ichiro Takamiya and Jos{\'e} Luis Vega Garrido and M. J. Pinto Zapata},
  journal={Analytical biochemistry},
  year={1995},
  volume={231 1},
  pages={
          225-9
        }
}
Most of the chlorophylls and their related compounds from nonesterified chlorophyllide to esterified pheophytin were separated by high- performance liquid chromatography using a wide pore, C(18) polyvinyl alcohol polymer column with an elution using a binary gradient starting from a buffered mobile phase. The high selectivity of this system enabled not only the separation of common chlorophyll species but also the resolution of structurally similar compounds such as mono- and divinyl… CONTINUE READING