Separation of mAbs molecular variants by analytical hydrophobic interaction chromatography HPLC: overview and applications

@inproceedings{Haverick2014SeparationOM,
  title={Separation of mAbs molecular variants by analytical hydrophobic interaction chromatography HPLC: overview and applications},
  author={Mark A. Haverick and Selina Mengisen and Mohammed Shameem and Alexandre Ambrogelly},
  booktitle={mAbs},
  year={2014}
}
Hydrophobic interaction chromatography-high performance liquid chromatography (HIC-HPLC) is a powerful analytical method used for the separation of molecular variants of therapeutic proteins. The method has been employed for monitoring various post-translational modifications, including proteolytic fragments and domain misfolding in etanercept (Enbrel®); tryptophan oxidation, aspartic acid isomerization, the formation of cyclic imide, and α amidated carboxy terminus in recombinant therapeutic… CONTINUE READING
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