Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.

@article{Veatch2003SeparationOL,
  title={Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.},
  author={Sarah L Veatch and Sebastian Keller},
  journal={Biophysical journal},
  year={2003},
  volume={85 5},
  pages={
          3074-83
        }
}
We use fluorescence microscopy to directly observe liquid phases in giant unilamellar vesicles. We find that a long list of ternary mixtures of high melting temperature (saturated) lipids, low melting temperature (usually unsaturated) lipids, and cholesterol produce liquid domains. For one model mixture in particular, DPPC/DOPC/Chol, we have mapped phase boundaries for the full ternary system. For this mixture we observe two coexisting liquid phases over a wide range of lipid composition and… CONTINUE READING

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