Separation of human immunoglobulin G subclasses on a protein A monolith column.

Abstract

Monolithic columns have attracted significant attention for the purification of large biomolecules. In the present study, a step gradient elution method was evaluated for the separation of human immunoglobulin G (hIgG) into its subclasses on CIM (convective interaction media) r-protein A (recombinant protein A) monolithic column. hIgG was loaded onto the… (More)
DOI: 10.1016/j.jchromb.2014.05.029

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