Separation of human IgA1 and IgA2 using jacalin-agarose chromatography.

@article{Gregory1987SeparationOH,
  title={Separation of human IgA1 and IgA2 using jacalin-agarose chromatography.},
  author={Richard Langton Gregory and J L Rundegren and Roland Ray Arnold},
  journal={Journal of immunological methods},
  year={1987},
  volume={99 1},
  pages={
          101-6
        }
}
A lectin isolated from the tropical jackfruit, jacalin, previously reported to precipitate human immunoglobulin A (IgA), and conjugated to agarose was used to separate the two subclasses of IgA from secretions. Jacalin-agarose binds specifically to the D-galactose moiety of IgA1 but not to IgA2 which has a different carbohydrate content and structure. IgA2 passed through the jacalin-agarose column and was collected in the void volume. IgA1 was eluted from the lectin by 0.8 M galactose. Of a… CONTINUE READING
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