Separation of glycopeptides by high performance liquid chromatography.

  title={Separation of glycopeptides by high performance liquid chromatography.},
  author={Marsha Rich Rosner and Phillips W. Robbins},
  journal={Journal of cellular biochemistry},
  volume={18 1},
A method is presented for separation of tryptic glycopeptides-containing oligosaccharides of the N-asparagine-linked type. High performance liquid chromatography (HPLC) of glycopeptides on a C18 reverse-phase system eluted with a gradient of 0%-50% acetonitrile in 0.1 M NaPO4 pH 2.2 resolves the two major glycosylation sites from the envelope glycoprotein (G) of vesicular stomatitis virus. Glycopeptides containing N-linked oligosaccharides of the complex type coelute with those containing N… CONTINUE READING

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