Separation of ferritin labeled antibody from free antibody.


A procedure for the separation of ferritin-antibody conjugates from free antibody has been developed using isokinetic sucrose density gradient sedimentation. The gradient (5% w/w sucrose at the top to about 35% at the tube bottom) provides optimal separation of 7S free immunoglobulin from the ferritin (65S) and ferritin-antibody conjugate. Recovery of the… (More)


Cite this paper

@article{Goldin1976SeparationOF, title={Separation of ferritin labeled antibody from free antibody.}, author={Eric M Goldin and Peter M. Corry and C. H. Granatek}, journal={Journal of immunological methods}, year={1976}, volume={13 3-4}, pages={299-303} }