Separation of extremely acidic proteins, S-100 proteins and calmodulin, in some bovine tissues and mammalian brains by two-dimensional electrophoresis in the absence of denaturing agents.

Extremely acidic proteins in bovine brain extracts, including S-100 proteins and calmodulin, were separated by two-dimensional electrophoresis in the absence of denaturing agents. The pattern was compared with the patterns of other bovine tissue extracts and also with those of brain extracts of other species. S-100 proteins (S-100 a and S-100 b) were… CONTINUE READING