Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

@article{Carle1984SeparationOC,
  title={Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.},
  author={G. F. Carle and Maynard V. Olson},
  journal={Nucleic acids research},
  year={1984},
  volume={12 14},
  pages={5647-64}
}
A simple agarose-gel apparatus has been developed that allows the separation of DNA molecules in the size range from 50 kb to well over 750 kb, the largest size for which size standards were available. The apparatus is based on the recent discovery that large DNA molecules are readily fractionated on agarose gels if they are alternately subjected to two approximately orthogonal electric fields. The switching time, which was on the order of 20-50 sec in our experiments, can be adjusted to… CONTINUE READING