Separation of caveolae from associated microdomains of GPI-anchored proteins

@article{Schnitzer1995SeparationOC,
  title={Separation of caveolae from associated microdomains of GPI-anchored proteins},
  author={Jenniffer Aparecida Schnitzer and DP McIntosh and Amanda Dvorak and J Liu and Phil Oh},
  journal={Science},
  year={1995},
  volume={269},
  pages={1435 - 1439}
}
In situ coating of the surface of endothelial cells in rat lung with cationic colloidal silica particles was used to separate caveolae from detergent-insoluble membranes rich in glycosyl phosphatidylinositol (GPI)-anchored proteins but devoid of caveolin. Immunogold electron microscopy showed that ganglioside GM1-enriched caveolae associated with an annular plasmalemmal domain enriched in GPI-anchored proteins. The purified caveolae contained molecular components required for regulated… 
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Flotillin and Epidermal Surface Antigen Define a New Family of Caveolae-associated Integral Membrane Proteins*
TLDR
Flotillin is a marker for the Triton-insoluble, buoyant membrane fraction in brain, where to date mRNA species for known caveolin gene family members have not been detected.
The tetraspan protein EMP2 modulates the surface expression of caveolins and glycosylphosphatidyl inositol-linked proteins.
TLDR
It is suggested that EMP2 facilitates the formation and surface trafficking of lipid rafts bearing GPI-APs, and reduces caveolin expression, resulting in impaired formation of caveolae.
Distinct interactions among GPI-anchored, transmembrane and membrane associated intracellular proteins, and sphingolipids in lymphocyte and endothelial cell plasma membranes.
Microdomains of GPI-anchored proteins in living cells revealed by crosslinking
TLDR
Chemical crosslinking is used to show that membrane microdomains of a GPI-anchored protein exist at the surface in living cells, which are much smaller than those seen after detergent extraction.
Cross-talk between Caveolae and Glycosylphosphatidylinositol-rich Domains*
TLDR
In cells lacking caveolae, the absence of GPI-anchored proteins caused an increase in cholesterol levels, suggesting a possible role in cholesterol homeostasis, which in some cells, such as Chinese hamster ovary cells, can be compensated by caveolin up-regulation.
Crowded little caves: structure and function of caveolae.
Human neutrophils are devoid of the integral membrane protein caveolin
TLDR
It is concluded that human neutrophils are devoid of caveolin and that secretory vesicles are not related to Caveolae nor dependent on caveolae for mobilization.
Endocytosis of lipid rafts: an identity crisis.
Biochemical analysis of a caveolae‐enriched plasma membrane fraction from rat liver
TLDR
It is concluded that the caveolae‐enriched plasma membrane fraction is a Plasma membrane fraction, which originates from specialized regions of the sinusoidal plasma membrane, enriched in Caveolae.
Lipid Raft and Platelet SNARE Machinery
TLDR
Using fluorescence microscopy of platelets being activated with thrombin and collagen, large fluorescent clusters of lipid rafts were formed, leading these investigators to conclude that raft aggregation is triggered by platelet activation, suggestive of a role for microdomains in platelet signaling.
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References

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TLDR
Analysis of the cell surface distribution of the GPI-anchored folate receptor by electron microscopy confirms that multimerization regulates their sequestration in caveolae, but in the absence of agents that promote clustering they are diffusely distributed over the plasma membrane.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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