Separation of Small DNA Fragments by Conventional Gel Electrophoresis

@article{Chory2001SeparationOS,
  title={Separation of Small DNA Fragments by Conventional Gel Electrophoresis},
  author={Joanne Chory and Jack D. Pollard},
  journal={Current Protocols in Molecular Biology},
  year={2001},
  volume={47}
}
  • J. Chory, J. Pollard
  • Published 1 July 1999
  • Biology, Chemistry
  • Current Protocols in Molecular Biology
Large amounts of small (<1000‐bp) DNA fragments can be separated by conventional electrophoretic means. The purified fragments can then be used for cloning, sequencing, and labeling. In this unit, the techniques of DNA separation via both nondenaturing polyacrylamide and sieving agarose electrophoresis are discussed. Methods are detailed for the pouring and electrophoresis of nondenaturing polyacrylamide gels, followed by elution of the labeled or unlabeled separated DNA fragments from the gels… 

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