Separation of Herpes simplex virus-induced antigens by Concanavalin A affinity chromatography.

  • M Ponce de Leon, H Hessle, G H Cohen
  • Published 1973 in Journal of virology

Abstract

Biologically active herpes simplex virus (HSV)-induced antigens were selectively removed from extracts of infected BHK cells by affinity chromatography by utilizing an insoluble form of concanavalin A (Con A). Soluble extracts of (3)H-glucosamine-labeled, HSV-infected cells were absorbed to a Con A column. Bound material was eluted with alpha-methyl-d-mannoside (alphaMM) and NaCl. The specific activity of the eluted glycoproteins increased by 10-fold. Two broad groups of viral-induced antigens were isolated from Con A. Group I includes two antigens which bind to Con A by a specific mechanism because the antigens are dissociated by alphaMM. Group II contains three antigens which bind to Con A but apparently by a nonspecific or electrolytic mechanism. One antigen in group I was identified as the glycoprotein antigen, CP-1, described previously.

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@article{Leon1973SeparationOH, title={Separation of Herpes simplex virus-induced antigens by Concanavalin A affinity chromatography.}, author={M Ponce de Leon and H Hessle and G H Cohen}, journal={Journal of virology}, year={1973}, volume={12 4}, pages={766-74} }