Separation of European flat oyster, Ostrea edulis, haemocytes by density gradient centrifugation and SDS-PAGE characterisation of separated haemocyte sub-populations.

@article{Xue2000SeparationOE,
  title={Separation of European flat oyster, Ostrea edulis, haemocytes by density gradient centrifugation and SDS-PAGE characterisation of separated haemocyte sub-populations.},
  author={Qinbin Xue and Tristan Renault and N Cochennec and Andr{\'e} G{\'e}rard},
  journal={Fish & shellfish immunology},
  year={2000},
  volume={10 2},
  pages={155-65}
}
A two-step gradient centrifugation with Percoll and Ficoll successively as density medium was developed to separate European flat oyster, Ostrea edulis, haemocytes into three sub-populations representing granulocytes, large hyalinocytes and small hyalinocytes, respectively. After a Percoll gradient centrifugation, granulocytes and agranulocytes were separated and a pure fraction of granulocytes was obtained. The agranulocytes were further separated by centrifugation through a Ficoll gradient… CONTINUE READING

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