Separation of E. coli expressing functional cell-wall bound antibody fragments by FACS.

@article{Fuchs1996SeparationOE,
  title={Separation of E. coli expressing functional cell-wall bound antibody fragments by FACS.},
  author={Patrick Fuchs and W Weichel and Stefan D{\"u}bel and Frank M Breitling and Melvyn Little},
  journal={Immunotechnology : an international journal of immunological engineering},
  year={1996},
  volume={2 2},
  pages={
          97-102
        }
}
BACKGROUND The rapid development of recombinant antibody technology in the last few years has facilitated the generation of antibody libraries in bacteria. Recombinant antibodies against various antigens have been selected from these libraries by presenting each antibody on the surface of a phagemid particle that contains the antibody's gene. An alternative screening system is the display of antibody fragments on bacteria. A major advantage is the possibility to select single cells directly… CONTINUE READING