Separation of Delta5- and Delta7-phytosterols by adsorption chromatography and semipreparative reversed phase high-performance liquid chromatography for quantitative analysis of phytosterols in foods.

@article{Zhang2006SeparationOD,
  title={Separation of Delta5- and Delta7-phytosterols by adsorption chromatography and semipreparative reversed phase high-performance liquid chromatography for quantitative analysis of phytosterols in foods.},
  author={Xin Zhang and Amandine Cambrai and Michel Miesch and Stamatiki Roussi and Françis Raul and Dalal Aoud{\'e}-Werner and Eric Marchioni},
  journal={Journal of agricultural and food chemistry},
  year={2006},
  volume={54 4},
  pages={1196-202}
}
A method for the separation, isolation, and identification of phytosterols was developed. A commercial phytosterols mixture, Generol 95S, was fractionated first by adsorption silica gel column chromatography and then separated by means of a semipreparative reverse phase high-performance liquid chromatography fitted with a Polaris C8-A column (250 mm x 10 mm i.d., 5 microm) using isocratic acetonitrile:2-propanol:water (2:1:1, v/v/v) as the mobile phase. Milligram scales of six individual… CONTINUE READING