Separation of 7 alpha- and 7 beta-hydroxysteroid dehydrogenase activities from clostridium absonum ATCC# 27555 and cellular response of this organism to bile acid inducers.

@article{Macdonald1983SeparationO7,
  title={Separation of 7 alpha- and 7 beta-hydroxysteroid dehydrogenase activities from clostridium absonum ATCC# 27555 and cellular response of this organism to bile acid inducers.},
  author={Ian Macdonald and Bryan A. White and Phillip B. Hylemon},
  journal={Journal of lipid research},
  year={1983},
  volume={24 9},
  pages={1119-26}
}
Both 7 alpha- and 7 beta-hydroxysteroid dehydrogenases (HSDH) were induced by either chenodeoxy-(CDC) or deoxycholic (DC) acid in C. absonum. 7 beta-HSDH was partially purified 35-fold from CDC-induced cultures of C. absonum by Procion Red (PR) affinity chromatography and high performance liquid chromatography (HPLC) using a TSK 3000 SW gel filtration column. A relative molecular weight of 200 K was estimated for 7 beta-HSDH using Sephacryl S-300 chromatography. Sodium dodecyl sulfate… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 20 references

Characterization of NADP - dependent 7 B - hydroxysteroid dehydrogenases from Peptostreptococcus productus and Eubacterium aerofa - cipns

  • N. Masuda
  • Appl . Environ . Microbwl .
  • 1982

Lyophilized 7 a - hydroxysteroid dehydrogenase : a stable enzyme preparation for routine bile acid analysis

  • M. C. Carey
  • J . Lipid Res
  • 1982

The metabolism of primary , 74 x 0 , and 7 B - hydroxy bile acids by Clostridium absonum

  • I. A. Macdonald
  • J . Lipui Res
  • 1982

Intracolonic conversion in man of chenodeoxycholic acid ( CDC ) to ursodeoxycholic acid ( UDC ) with and without formation of 7 - keto - lithocholic acid as an intermediate

  • R. P. Sarva, H. Fromm, G. L. Carlson, L. Mendelow, S. Ceryak
  • Gastroenterology
  • 1980

The specificity of a 7 a - hydroxysteroid dehydrogenase from Escherichia coli

  • P. D. G Dean
  • Biochem . J .
  • 1979

Similar Papers

Loading similar papers…