Separation and recovery of DNA fragments by electrophoresis through a thermoreversible hydrogel composed of poly(ethylene oxide) and poly(propylene oxide).

@article{Yoshioka2003SeparationAR,
  title={Separation and recovery of DNA fragments by electrophoresis through a thermoreversible hydrogel composed of poly(ethylene oxide) and poly(propylene oxide).},
  author={Hiroshi Yoshioka and Yuichi Mori and Mitsuhiro Shimizu},
  journal={Analytical biochemistry},
  year={2003},
  volume={323 2},
  pages={218-23}
}
We have synthesized and characterized a thermoreversible hydrogel of multiplied block copolymers, composed of poly(ethylene oxide) and poly(propylene oxide), for DNA electrophoresis. The aqueous solution of block copolymers turned into a hydrogel upon heating at temperatures above 10-11 degrees C, whereas it reverted into a solution upon cooling. Linear… CONTINUE READING