Separation and quantitation of nitrobenzenes and their reduction products nitroanilines and phenylenediamines by reversed-phase high-performance liquid chromatography

  title={Separation and quantitation of nitrobenzenes and their reduction products nitroanilines and phenylenediamines by reversed-phase high-performance liquid chromatography},
  author={T. Reddy and B. Wiechman and E. Lin and L. Chang and M. K. Smith and F. Daniel and G. Reddy},
  journal={Journal of Chromatography A},
Abstract A reversed-phase high-performance liquid chromatographic method for the separation and quantitation of a mixture consisting of nitrobenzene, dinitrobenzene isomers, 1,3,5-trinitrobenzene and their reduction products: aniline, nitroanilines and phenylenediamines has been developed. The method is sensitive and highly reproducible. The mixture is resolved on a Zorbax C 8 column with 0.1% triethylamine and methanol as the mobile phase. The detection limits for individual chemicals at 254… Expand
Simultaneous determination of oxidative hair dye p-phenylenediamine and its metabolites in human and rabbit biological fluids.
A liquid chromatography with an electrochemical detector method has been developed for the quantitative measurement for three diamine derivatives in human urine and rabbit blood, urine, and feces. Expand
Separation and determination of nitroaniline isomers by capillary zone electrophoresis with amperometric detection.
Capillary zone electrophoresis with amperometric detection (CZE-AD) was firstly applied to the simultaneous separation and determination of nitroaniline positional isomers and demonstrated long-term stability and reproducibility with relative standard deviations of less than 1.1%. Expand
Separation and determination of aminophenols and phenylenediamines by liquid chromatography and micellar electrokinetic capillary chromatography
Abstract The separation and determination of aminophenols and phenylenediamines were investigated by liquid chromatography (LC) and micellar electrokinetic chromatography (MEKC) in this study.Expand
A simple colorimetric method for analysis of aqueous phenylenediamines and aniline
A simple and accurate method has been developed for the field and laboratory analysis of four highly toxic substances found in certain industrial wastes in high concentrations of phenylenediamine, aniline, trinitrobe, and 1,4-phenylenedienine. Expand
Use of porous polymers in off-line preconcentration of nitrobenzenes and their reduction products from water
Abstract Porous polymers with different functional groups were tested as sorbents for off-line preconcentration of nitrobenzenes together with their reduction products from water. In addition toExpand
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In order to determine the simultaneous presence of chlorobenzenes and nitrobenzenes in marine sediments, a simple extraction method by an automatic extraction system was developed, which allowed the extraction of the analytes of interest with no waste of volatile compounds. Expand
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Nitrobenzenes, such as the antibiotic chloramphenicol, the vasodilator nicardipine, and the herbicides dinitramin, dinobuton, fenitrothion, methylparathion, oxyfluorfen, parathion, pendimethalin,Expand
Reactions of reducing radicals with 2- and 3-nitroanilines in aqueous solutions: a pulse radiolysis study
Reactions of 2- and 3-nitro anilines (2- and 3-NA) with eaq−, H-atoms and one-electron reductants have been studied using pulse radiolysis in aqueous solutions. Reactions of eaq− were found to beExpand
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Abstract The influence of H 2 O 2 and Fe(III) in the degradation of nitrobenzene in aqueous solutions combined with UV radiation has been studied. A major efficiency is achieved when UV is combinedExpand


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Abstract N-Propylanilinetrimethoxysilane was reacted with 10 μm silica and the resulting bonded stationary phase was assessed for the liquid chromatographic separation of nitro and polynitroaromaticExpand
Separation of mixtures of 2,4,6-trinitrotoluene reduction products with liquid chromatography
Abstract A high-performance liquid Chromatographic procedure is described for the partial separation of 2,4,6-trinitrotoluene and eight reduction products. A methanol gradient effectively separatesExpand
Reversed-phase high-performance liquid chromatographic determination of nitroorganics in munitions wastewater
Concentrations of HMX, RDX, TNT, and 2,4-DNT are determined in munitions wastewater. Aqueous samples are diluted with an equal volume of 76/24 (v/v) methanol-acetonitrile, filtered through aExpand
Trace analysis of explosives by thermospray high-performance liquid chromatography—mass spectrometry
Abstract A series of explosives were analyzed by thermospray high-performance liquid chromatography—mass spectrometry (TSP-HPLC—MS). Both positive and negative ion spectra were acquired inExpand
Microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds
A variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (TNT), were reduced by hydrogen in the presence of enzyme preparations from Veillonella alkalescens, indicating the question of whether ferredoxin acts as a nonspecific reductase for nitroARomatic compounds remains unresolved. Expand
Metabolism of dinitrobenzenes by rat isolated hepatocytes.
  • P. Cossum, D. Rickert
  • Chemistry, Medicine
  • Drug metabolism and disposition: the biological fate of chemicals
  • 1985
There are substantial isomeric differences in the aerobic metabolism of the DNBs by rat hepatic enzymes in hepatocytes and hepatic subcellular fractions isolated from male Fischer-344 rats. Expand
Metabolism and toxicity of dinitrobenzene isomers in erythrocytes from Fischer-344 rats, rhesus monkeys and humans.
The data suggest that although the erythrocyte can conjugate DNB isomers with glutathione, this pathway offers no substantial protection from methemoglobinemia induced by dinitrobenzenes. Expand
The involvement of the gastro-intestinal microflora in nitro-compound-induced methaemoglobinaemia in rats and its relationship to nitrogroup reduction.
Investigations into the methaemoglobinaemia-inducing capacity of three mono- and five dinitro-compounds in both normal and antibiotically pretreated rats in vivo, and in vitro on incubation with rat blood suggest that in some cases the conversion of haemoglobin to meethaemoglobin could occur possibly as a result of a direct interaction between unchanged nitro-Compounds and haememia. Expand
Comparison of extraction techniques for munitions residues in soil
Extraction of trinitrotoluene (TNT), trinitrobenzene (TNB), hexahydrotrinitrotriazine (RDX), and octahydrotetranitrotetrazocine (HMX) from two soils was studied in terms of process kinetics andExpand
Genotoxic activity in microorganisms of tetryl, 1,3-dinitrobenzene and 1,3,5-trinitrobenzene.
In this segment of a programme initiated by military authorities, the genotoxic potential of three nitroaromatic compounds, which have found significant use in explosive preparations, has been demonstrated. Expand