Separation and quantification of the "middle molecules" in uremia.

  title={Separation and quantification of the "middle molecules" in uremia.},
  author={G V Chapman and Richard A. Ward and Professor Geoffrey C. Farrell},
  journal={Kidney international},
  volume={17 1},
A modification of a two-stage chromatographic procedure (molecular sieve followed by ion-exchange) to separate potentially toxic uremic "middle molecules" from body fluids has been established. The procedure has an analysis time less than half that previously reported with improved resolution. Elution volumes have been found to be reproducible to within 2% and concentrations (as measured by peak height) to within 10%. Careful attention, however, must be paid to artifacts that may arise from… CONTINUE READING