Separation and purification of liver microsomal monooxygenases from induced and untreated pigs.

@article{Tsuji1980SeparationAP,
  title={Separation and purification of liver microsomal monooxygenases from induced and untreated pigs.},
  author={Hiromitsu Tsuji and E Muta and Volker Ullrich},
  journal={Hoppe-Seyler's Zeitschrift fur physiologische Chemie},
  year={1980},
  volume={361 5},
  pages={681-96}
}
From liver microsomes of phenobarbital-, beta-naphthoflavone- and ethanol-pretreated male pigs and of controls NADPH-cytochrome P-450 reductase and four cytochrome P-450 fractions were isolated according to a modified procedure of Imai and Sato. Electrophoretic, immunological, spectral and catalytic properties suggested that at least five different forms of cytochrome P-450 could be separated, two of which were obtained in a pure state. Using selective inhibitors and antisera it was concluded… CONTINUE READING