• Chemistry, Medicine
  • Published in
    Journal of agricultural and…
    2000
  • DOI:10.1021/jf990876t

Separation and purification of anthocyanins by high-speed countercurrent chromatography and screening for antioxidant activity.

@article{Degenhardt2000SeparationAP,
  title={Separation and purification of anthocyanins by high-speed countercurrent chromatography and screening for antioxidant activity.},
  author={Andreas Degenhardt and Holger Knapp and Peter Winterhalter},
  journal={Journal of agricultural and food chemistry},
  year={2000},
  volume={48 2},
  pages={
          338-43
        }
}
The all-liquid chromatographic technique of high-speed countercurrent chromatography (HSCCC) has been applied for separations of anthocyanins. The biphasic mixture of tert-butyl methyl ether/n-butanol/acetonitrile/water (2:2:1:5) acidified with trifluoroacetic acid was found to be a suitable solvent system for anthocyanin separation. In some cases, enrichment of the pigments on Amberlite XAD-7 resin prior to HSCCC has been carried out. The anthocyanin mixtures from red cabbage, black currant… CONTINUE READING

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