Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T–seq

@article{Macaulay2016SeparationAP,
  title={Separation and parallel sequencing of the genomes and transcriptomes of single cells using G\&T–seq},
  author={Iain C. Macaulay and Mabel J Teng and Wilfried Haerty and Parveen Kumar and Chris Paul Ponting and Thierry Voet},
  journal={Nature Protocols},
  year={2016},
  volume={11},
  pages={2081-2103}
}
Parallel sequencing of a single cell's genome and transcriptome provides a powerful tool for dissecting genetic variation and its relationship with gene expression. Here we present a detailed protocol for G&T-seq, a method for separation and parallel sequencing of genomic DNA and full-length polyA(+) mRNA from single cells. We provide step-by-step instructions for the isolation and lysis of single cells; the physical separation of polyA(+) mRNA from genomic DNA using a modified oligo-dT bead… 
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