Separation and isolation of trace impurities in L-tryptophan by high-performance liquid chromatography.

@article{Trucksess1993SeparationAI,
  title={Separation and isolation of trace impurities in L-tryptophan by high-performance liquid chromatography.},
  author={Mary W. Trucksess},
  journal={Journal of chromatography},
  year={1993},
  volume={630 1-2},
  pages={147-50}
}
A high-performance liquid chromatographic (HPLC) profiling method was developed to separate trace impurities in L-tryptophan products associated with the eosinophilia-myalgia syndrome (EMS) epidemic. The test portion was dissolved in water, and the solution was filtered and chromatographed on a silica-based C18 reversed-phase HPLC column by using linear gradient elution with water and acetonitrile-water (80:20); both solvents contained 0.1% trifluoroacetic acid for ion-pairing. The method was… CONTINUE READING