Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTLC-DPPH method.

@article{Pozharitskaya2008SeparationAF,
  title={Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTLC-DPPH method.},
  author={Olga N. Pozharitskaya and Svetlana A. Ivanova and Alexander N Shikov and Valery G Makarov},
  journal={Phytochemical analysis : PCA},
  year={2008},
  volume={19 3},
  pages={
          236-43
        }
}
A direct HPTLC assay was developed for the determination of total curcuminoids and three individual curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin. In addition, a new procedure was developed to separate and quantitative the free radical-scavenging activity of individual compounds from the rhizome of Curcuma longa L. (Zingiberaceae) based on the combination of HPTLC with a diode array detector (DAD) and post chromatographic DPPH(*) radical derivatisation. It was established… CONTINUE READING
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