Separation and determination of phospholipids in biological samples by high-performance liquid chromatography.

@article{Wang2003SeparationAD,
  title={Separation and determination of phospholipids in biological samples by high-performance liquid chromatography.},
  author={Fengjun Wang and Yun Zhao and Pei Wang},
  journal={Journal of chromatographic science},
  year={2003},
  volume={41 3},
  pages={142-4}
}
An isocratic high-performance liquid chromatographic method is developed for the determination of phospholipids in biological samples using a muPorasil silica column and a mobile phase of acetonitrile-methanol-85% phosphoric acid (90:3:1, v/v/v) at a flow rate of 0.80 mL/min. The effluent is monitored by a UV detector at 203 nm. With the method reported in this paper, phosphatidylinostol, phosphatidylserine, phosphatidylethnolamine, and phosphatidylcholine in biological samples are separated… CONTINUE READING

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