Separation and characterization of the troponin components from bovine cardiac muscle.

@article{Brekke1976SeparationAC,
  title={Separation and characterization of the troponin components from bovine cardiac muscle.},
  author={C J Brekke and M. L. Greaser},
  journal={The Journal of biological chemistry},
  year={1976},
  volume={251 3},
  pages={866-71}
}
The three major components of bovine cardiac troponin were separated by successive chromatography on sulfopropyl-Sephadex and DEAE-Sephadex columns in the presence of 6 M urea. All three of the bovine cardiac troponin subunits were necessary to restore full troponin activity in both skeletal and cardiac actomyosin ATPase assay systems. The 38,000-dalton… CONTINUE READING