Separation and characterization of the complexes constituting the cellulolytic enzyme system of Clostridium thermocellum

@article{Honnami2004SeparationAC,
  title={Separation and characterization of the complexes constituting the cellulolytic enzyme system of Clostridium thermocellum},
  author={Koyu Hon-nami and Michael P. Coughlan and Hiromi Hon-nami and Lars G. Ljungdahl},
  journal={Archives of Microbiology},
  year={2004},
  volume={145},
  pages={13-19}
}
Clostridium thermocellum, strain JW20 (ATCC 31449) when growing in cellulose produces a cellulolytic enzyme system, that at the early stage of the fermentation is largely bound to the substrate. As cellulose is consumed the bound enzyme is released as free enzyme to the culture fluid. The bound enzyme fraction extracted with distilled water from the cellulose contains two major components, a large complex (Mr≃100×106) and a small complex Mr≃4.5×106) which were separated by gel filtration and… CONTINUE READING
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