Separation and characterization of high-density lipoprotein subpopulations by gel permeation chromatography.

@article{Clifton1987SeparationAC,
  title={Separation and characterization of high-density lipoprotein subpopulations by gel permeation chromatography.},
  author={P M Clifton and A M Mackinnon and Philip J. Barter},
  journal={Journal of chromatography},
  year={1987},
  volume={414 1},
  pages={25-34}
}
High-density lipoproteins (HDL) contain at least five distinct subpopulations when analyzed by gradient gel electrophoresis. This report represents the first description of a simple technique for isolating these subpopulations of HDL in quantities sufficient to enable characterization in terms of particle size, apolipoprotein AI and apolipoprotein AII content and chemical composition. Lipoproteins were separated and subfractionated on a column of Superose 6B using a fast protein liquid… CONTINUE READING

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