Separating homeologs by phasing in the tetraploid wheat transcriptome

Abstract

The high level of identity among duplicated homoeologous genomes in tetraploid pasta wheat presents substantial challenges for de novo transcriptome assembly. To solve this problem, we develop a specialized bioinformatics workflow that optimizes transcriptome assembly and separation of merged homoeologs. To evaluate our strategy, we sequence and assemble… (More)
DOI: 10.1186/gb-2013-14-6-r66

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