Separate pathways for cellular uptake of ferric and ferrous iron.

@article{Conrad2000SeparatePF,
  title={Separate pathways for cellular uptake of ferric and ferrous iron.},
  author={Marcel E. Conrad and Jay N. Umbreit and Elizabeth G. Moore and Lucille N. Hainsworth and Michael Porubcin and Marcia J. Simovich and Marian T. Nakada and Kevin G. Dolan and Michael D. Garrick},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  year={2000},
  volume={279 4},
  pages={
          G767-74
        }
}
  • M. Conrad, J. Umbreit, +6 authors M. Garrick
  • Published 1 October 2000
  • Biology, Chemistry
  • American journal of physiology. Gastrointestinal and liver physiology
Separate pathways for transport of nontransferrin ferric and ferrous iron into tissue cultured cells were demonstrated. Neither the ferric nor ferrous pathway was shared with either zinc or copper. Manganese shared the ferrous pathway but had no effect on cellular uptake of ferric iron. We postulate that ferric iron was transported into cells via beta(3)-integrin and mobilferrin (IMP), whereas ferrous iron uptake was facilitated by divalent metal transporter-1 (DMT-1; Nramp-2). These… 
View on PubMed
ajpgi.physiology.org
133 Citations
Highly Influential Citations
7
Background Citations
73
Methods Citations
4
Results Citations
4

Figures and Tables from this paper

133 Citations

Citation Type

Citation Type

The ferrireductase paraferritin contains divalent metal transporter as well as mobilferrin.
TLDR
Findings suggest a role for DMT-1 in conveyance of iron from paraferritin to ferrochelatase, the enzyme utilizing ferrous iron for the synthesis of heme in the mitochondrion.
Uptake of ferrous iron by cultured rat astrocytes
TLDR
Data indicate that, in addition to the DMT1‐mediated uptake of ferrousIron, astrocytes can accumulate ferric and ferrous iron by mechanisms that are independent of D MT1 or transferrin.
Effects of Iron Chelators, Iron Salts, and Iron Oxide Nanoparticles on the Proliferation and the Iron Content of Oligodendroglial OLN-93 Cells
TLDR
Proliferation of OLN-93 cells depends strongly on the availability of iron and that these cells efficiently accumulate iron from various extracellular iron sources, although the cells efficiently accumulated iron during exposure to each of these iron sources.
Iron transport across cell membranes: molecular understanding of duodenal and placental iron uptake.
TLDR
Recently identified transmembrane transporters and associated accessory proteins responsible for iron transport at two sites are described, including the placento-fetal barrier where similar principles operate.
Pathways of iron absorption.
TLDR
Iron uptake by intestinal absorptive cells is regulated by the iron concentration within the cell, and except in hemochromatosis it remains in equilibrium with total body stores via transferrin receptors on the basolateral membrane of absorptive Cells.
Iron absorption and transport—An update
TLDR
SFT (Stimulator of Iron Transport) is postulated to facilitate both ferric and ferrous iron uptake, and Hephaestin is thought to be important in transfer of iron from enterocytes into the plasma, suggesting that these pathways have intracellular functions in addition to facilitating iron uptake.
Effects of copper and ceruloplasmin on iron transport in the Caco 2 cell intestinal model.
A role for divalent metal transporter (DMT1) in mitochondrial uptake of iron and manganese
TLDR
A role for DMT1 is supported in mitochondrial Fe2+ and Mn2+ acquisition and transport was defective in rKC mitochondria with the Belgrade (G185R) mutation.
Stimulation of non-transferrin iron uptake by iron deprivation in K562 cells.
Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 48 REFERENCES
Characterization of a transferrin-independent uptake system for iron in HeLa cells.
Alternate iron transport pathway. Mobilferrin and integrin in K562 cells.
Mechanism of Iron Transport to the Site of Heme Synthesis inside Yeast Mitochondria*
TLDR
By measuring the incorporation of iron into porphyrin by ferrochelatase in the matrix, the mechanism of iron import into yeast mitochondria is defined and the basis for the identification of components involved in transport is provided.
Regulation of the transferrin-independent iron transport system in cultured cells.
Membrane transport of non-transferrin-bound iron by reticulocytes.
  • E. Morgan
  • Biology, Computer Science
    Biochimica et biophysica acta
  • 1988
Non-transferrin-bound iron uptake by rat liver. Role of membrane potential difference.
TLDR
All effects were reversible, suggesting that perfusion with 1 microM iron produced little toxicity, consistent with an electrogenic transport mechanism for uptake of non-transferrin-bound iron that is driven by the transmembrane potential difference.
Iron absorption and cellular transport: the mobilferrin/paraferritin paradigm.
TLDR
It is postulate that the basolateral membranes of absorptive cells possess both holo-transferrin and apotransferrin receptors that regulate the ingress and egress of cellular iron, respectively, and two transferrin-associated pathways donate iron to a common intracellular iron pathway for ferri-reduction and probably other functions.
Inhibition of uptake of transferrin‐bound iron by human hepatoma cells by nontransferrin‐bound iron
TLDR
The results suggest that the uptake of NTB‐Fe and Tf‐Fe by the NTR‐mediated process occurs by the same cellular pathway, using a common iron‐carrier.
Characterisation of citrate and iron citrate uptake by cultured rat hepatocytes.
Iron transport mechanisms in reticulocytes and mature erythrocytes
TLDR
It is concluded that erythroid cells can take up nontransferrin‐bound Fe(II) by two mechanisms, a high‐affinity mechanism that is limited to reticulocytes, saturates at a low iron concentration, and is inhibited by metabolic inhibitors, and a low-affinity process that is found in both reticULocytes and erythrocytes, becomes more prominent at higher iron concentrations.
...
1
2
3
4
5
...