Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis.

@article{Perkins2001SeparateSR,
  title={Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis.},
  author={G. R. Perkins and Lucy S. Drury and John F. X. Diffley},
  journal={The EMBO journal},
  year={2001},
  volume={20 17},
  pages={4836-45}
}
The Cdc6 DNA replication initiation factor is targeted for ubiquitin-mediated proteolysis by the E3 ubiquitin ligase SCF(CDC4) from the end of G1phase until mitosis in the budding yeast Saccharomyces cerevisiae. Here we describe a dominant-negative CDC6 mutant that, when overexpressed, arrests the cell cycle by inhibiting cyclin-dependent kinases (CDKs) and, thus, prevents passage through mitosis. This mutant protein inhibits CDKs more efficiently than wild-type Cdc6, in part because it is… CONTINUE READING

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