Separate Determination by Gas-Chromatography of Dimethylformamide, Dimethylacetamide, Monomethylformamide and Monomethylacetamide in Urine for Biological Monitoring*

Abstract

Separate Determination by GasC h r o m a t o g r a p h y o f D i m e t h y l f o r m a m i d e , Dimethylacetamide, Monomethylformamide and Monomethylacetamide in Urine for Biological Monitoring: Toshio KAWAI, et al. Osaka Occupational Health Service Center—A gaschromatographic method is developed, which allows simultaneous determination o f N,N d ime thy l f o rmamide (DMF) and N,N dimethylacetamide (DMA), as well as their metabolites of N m o n o m e t h y l f o r m a m i d e ( M M F ) a n d N monomethylacetamide (MMA), respectively. Quantitative h e a t d e c o m p o s i t i o n o f N h y d r o x y m e t h y l N methylacetamide to N-monomethylacetamide required an injection port temperature of 225°C or above, similar to the case of conversion from N-hydroxymethyl-Nmethylformamide to N-monomethylformamide. Analysis of urine samples from workers simultaneously exposed to DMF and DMA showed that the separation of small peaks for unidentified materials (which were detected in some urine samples) from that of DMF and DMA was achieved only on a 60 m-long DB-1071 column (and not on a 30 m-long one). The analysis of urine samples from 27 exposed workers showed that both DMF and DMA, in addition to MMF and MMA, were excreted in urine at measurable concentrations. There was a significant correlation between MMF and DMF, and between MMA and DMA; the ratio was about 0.02 to 0.03 for both DMF/ MMF and DMA/MMA. (J Occup Health 1997; 39: 113–118) Received July 8, 1996; Accepted Sept 30, 1996 Correspondence to: M. Ikeda. Present address; Kyoto Industrial Health Association, 67 Nishinokyo-Kitatsuboicho, Nakagyo-ku, Kyoto 604,

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Cite this paper

@inproceedings{Kawai2001SeparateDB, title={Separate Determination by Gas-Chromatography of Dimethylformamide, Dimethylacetamide, Monomethylformamide and Monomethylacetamide in Urine for Biological Monitoring*}, author={Toshio Kawai and Kazunori Mizunuma and Yoko Okada and T Odachi and S Horiguchi and Hiroshi Iguchi and Masayuki Ikeda}, year={2001} }