Sentinel Node Biopsies in Melanoma Patients: A Protocol for Accurate, Efficient, and Cost-Effective Analysis by Preselection for Immunohistochemistry on the Basis of Tyr-PCR

@article{VeldeZimmermann2000SentinelNB,
  title={Sentinel Node Biopsies in Melanoma Patients: A Protocol for Accurate, Efficient, and Cost-Effective Analysis by Preselection for Immunohistochemistry on the Basis of Tyr-PCR},
  author={PhD D. van der Velde-Zimmermann and Marguerite E. I. Schipper and PhD R. A. de Weger and PhD A. Hennipman and PhD I. H. M. Borel Rinkes},
  journal={Annals of Surgical Oncology},
  year={2000},
  volume={7},
  pages={51-54}
}
Background: Immunohistochemistry (IHC) of serial sectioning is considered the gold standard for detection of melanoma activity in sentinel node (SN) biopsies. However, this is cost and labor intensive. In contrast, tyrosinase reverse transcription-polymerase chain reaction (RT-PCR) is simple and quick, but it is hampered by its extreme sensitivity. This study was performed to test whether a strategy that combines the two methods, using tyrosinase RT-PCR to preselect nodes for IHC, could be… CONTINUE READING