Sensor of phospholipids in Streptomyces phospholipase D.

  title={Sensor of phospholipids in Streptomyces phospholipase D.},
  author={Yoshiko Uesugi and Jiro Arima and Masaki Iwabuchi and Tadashi Hatanaka},
  journal={The FEBS journal},
  volume={274 10},
Recently, we identified Ala426 and Lys438 of phospholipase D from Streptomyces septatus TH-2 (TH-2PLD) as important residues for activity, stability and selectivity in transphosphatidylation. These residues are located in a C-terminal flexible loop separate from two catalytic HxKxxxxD motifs. To study the role of these residues in substrate recognition, we evaluated the affinities of inactive mutants, in which these residues were substituted with Phe and His, toward several phospholipids by SPR… CONTINUE READING